Ku protein.

The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku70 (p70) and Ku86 (p86) which is also known by the synonym Ku80 or (p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma polymyositis overlap syndrome, and was later found in the sera of patients with ...Services Offered: Protein construct design, expression and purification for crystallization (gene-to-structure) Protein crystallization (only 12 mL of protein needed per 96-well screen) High throughput methods using robotics for crystallization and crystal imaging. Screening of protein ligand, inhibitor complexes and protein:protein complexes. The Ku protein consists of two tightly associated homologous subunits, Ku70 and Ku80, and was originally identified as an autoantigen recognized by the sera of patients with an autoimmunity disease. In eukaryotes, the Ku heterodimer contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by ... Human Ku heterodimeric protein composed of Ku70 and Ku80 subunits plays an important role in the non-homologous end-joining DNA repair pathway as a sensor of double strand DNA breaks. Ku is also involved in numerous cellular processes, and in some of them it acts in an RNA-dependent manner. However, …

The current thinking is that Ku protein binds to DNA discontinuously and in a sequence independent manner, carrying out a DNA-protective role . Once bound to DNA, Ku proteins recruit and activate the catalytic DNAPKcs subunit which can phosphorylate Ku and other neighboring DNA-bound proteins . It has also been reported that DNAPKcs self ...Polyomavirus enhancer-binding protein 2/core binding factor/acute myeloid leukemia factors contribute to the cell type-specific activity of the CD11a integrin gene promoter. ... Heat shock transcription factor 1 binds selectively in vitro to Ku protein and the catalytic subunit of the DNA-dependent protein kinase. The Journal of biological ...

Autoantibodies targeting the Ku antigen have been reported in patients with antinuclear antibody (ANA) associated rheumatic diseases (AARDs) 1-3 such as systemic lupus erythematosus (SLE), autoimmune myositis (AIM) 4 and systemic sclerosis (SSc). 5 The Ku autoantigen is a heterodimeric protein comprising of two subunits (Ku70 and Ku80) with sequence specific binding affinity for DNA 6 and to ...Protein dimerization controls many physiological processes in the body. Proteins form homo-, hetero-, or oligomerization in the cellular environment to regulate the cellular processes. ... The bridge-region of the Ku superfamily is an atypical zinc ribbon domain. Journal of Structural Biology, 172 (3), 294-299. 10.1016/j.jsb.2010.05.011.

Ku protein binds to DNA ends and is a cofactor for the DNA-dependent protein kinase. Both of these components are involved in DNA double-strand break repair, but it has not been clear if they function indirectly, by sensing DNA damage and activating other factors, or if they are more directly involved in the processing and rejoining of DNA breaks.Here, we report that the Ku proteins (Ku80 and Ku70) directly interact with cGAS and positively regulate cGAS-mediated antiviral signaling. Mechanistically, we find that the interaction of the Ku proteins with cGAS significantly increases the DNA-binding affinity of cGAS and promotes cGAS condensation in the cytosol, thereby enhancing cGAS ...While Ku binds the initial break and recruits LigD, it is LigD that is the primary DNA end processing machinery. Up to three enzymatic domains reside within LigD, dependent on the bacterial species.Other DOCKGROUND publications: Douguet D, Chen H, Tovchigrechko A, Vakser IA Dockground resource for studying protein-protein interfaces. Bioinformatics 2006, 22:2612-2618. Gao Y, Douguet D, Tovchigrechko A, Vakser IA Dockground system of databases for protein recognition studies: Unbound structures for docking. Proteins 2007, 69:845-851.Here, a 3D structural model of the full-length Ku protein is proposed on the basis of single-particle EM experiments. Results Ku purification and its interaction with DNA. Ku70–Ku80 was purified from HeLa cells . The ability of our preparation to form a stable complex with DNA was tested by incubation with a 54 bp oligonucleotide.

KU-60019是一种改进的KU-55933类似物，在无细胞试验中作用于ATM，IC50为6.3 nM，作用于ATM比作用于DNA-PK和ATR的选择性分别高270和1600倍，并且是高度有效的放射增敏剂。

May 17, 2017 · DNA-dependent protein kinase catalytic subunit (DNA-PKcs) has a high affinity for Ku–DNA ends and, together with Ku, forms the DNA-PK complex 4 . Figure 1: Overview of non-homologous end joining.

(C) Electrophoretic mobility shift assay of radiolabeled ds DNA probe incubated with purified human recombinant Ku protein in the presence of various competitor ssO bearing a biotin moiety at the 3′-, 5′- or 3′- and 5′- end, blocked or not by streptavidin, as indicated. ds probe and ssO amount in reactions were 0.2 and 10 pmol ...Ku is found throughout eukaryotic evolution and in higher eukaryotes is associated with the DNA-dependent protein kinase catalytic subunit (DNA-PK cs) to form the DNA-PK holoenzyme. Recently,...Ka'Chava contains 25 grams of protein per 2-scoop serving. Our protein comes from a variety of high-quality plant-based sources (pea protein, sacha inchi, brown rice, amaranth, quinoa) to ensure you get the appropriate ratios of all nine essential amino acids your body needs to thrive. Most nutrition experts recommend consuming around 25-30 ...Oxidative stress induces DNA damage which can be repaired by DNA repair proteins, such as Ku70/80. Excess reactive oxygen species (ROS) stimulate the activation of caspase-3, which degrades Ku 70/80. Cells with decreased Ku protein levels undergo apoptosis. Astaxanthin exerts antioxidant activity by inducing the expression of catalase, an antioxidant enzyme, in gastric epithelial cells ...KU80 was one of the first radiosensitivity genes discovered from such studies (Taccioli et al., 1994 ). Its function is to protect the ends of DNA from further degradation and, importantly, to recruit the protein kinase DNA-PKcs (DNA-dependent protein kinase catalytic subunit (officially known as PRKDC)).

This means that a 1 mg/ml solution of the protein will have an absorbance at 280 nm of 0.54. E(0.1%, 280) can be used as a very convenient measure of the protein content provided that the enzyme preparation supplied by Sigma is pure. A 'rule of thumb', useful when the E(0.1%, 280) is unknown, is that a 1mg/ml protein solution has an A 280 of 1. The protein expression of Ku 70 and Ku 80 was closely related and decreased consistently with increasing grades and stages, paralleling the expression of the mRNA. Among hazardous environmental bladder cancer risk factors, heavy consumption of coffee was associated with a twofold decreased Ku 70 and Ku 80 mRNA expression, whereas tobacco smoke ...Ku70 and Ku80 form a heterodimer, Ku, which possesses DNA end-binding activity (Mimori and Hardin, 1986). Purified Ku protein was found to promote the association of two DNA molecules in vitro; thus, it was proposed to possess end bridging or alignment activity (Ramsden and Gellert, 1998). In 1994 the first human autoantigen (Ku protein) was identified as DNA helicase [25]. In 1996 Tuteja and his colleagues reported the first plant DNA helicase in the purified form from pea chloroplast [26] and Subramanya and his colleagues solved the crystal structure of the first DNA helicase (PcrA from thermophilic bacterium) [27].Yeast KU protein 1 Comparative Info. Sequence Details Sequence The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains. ...Composed of the DNA-PK catalytic subunit (DNA-PKcs), a PI3K-related protein kinase, and the Ku70/80 heterodimer (Ku), this large protein complex assembles on exposed DNA termini to become an active protein kinase that is, in general, unique to vertebrates . Ku is composed of one 70 kDa (Ku70) subunit and one 86 kDa (Ku80) subunit.In a recent study published in Cell Reports, researchers from the Institute of Zoology of the Chinese Academy of Sciences and Yunnan University revealed that Ku proteins act as co-sensors of cGAS ...

Protein Modeling—The SPOCK modeling program (25) was used to dock the crystal structures of the core nucleosome (Protein Data Bank accession 1AOI) and Ku bound to DNA (accession 1JEY) by multiple alignments of the phosphodiester backbone of residues 2-12 in chain C of 1JEY with the backbone of 10 consecutive residues in chain I of 1AOI.Iterative alignments were made, advancing target ...

S1). In contrast to eukaryotes, mycobacteria such as Mycobacteria marinum possess only a single small Ku protein of 291 amino acids which forms a Ku homodimer for binding DNA ends and NHEJ repair ...6 Mar 2019 ... Al Awwaly, K.U. 2017. Protein Pangan Hasil Ternak dan Aplikasinya. Universitas. Brawijaya Press, Malang. Anonim. Bikin Es Krim. https ...Many of the genes involved in NHEJ in higher organisms have been identified and their protein products characterised (reviewed in [2]).The first clue that bacteria also repair double-stranded DNA breaks by NHEJ was the identification of a bacterial homologue of the mammalian repair protein, Ku 3., 4..Interestingly, whereas eukaryotic organisms encode two Ku proteins, Ku70 and Ku80, the ...May 20, 2023 · Ku protein possesses a ds DNA end binding activity, it also has a major role in V(D)J recombination. The importance of DNA helicases is further strengthened b y the dis­ covery that the Bloom's ...Conventionally, KU complex, the regulatory subunit of DNA-dependent protein kinase (DNA-PK), together with the catalytic subunit of DNA-PK (DNA-PKcs), mediates DNA damage repair in the nucleus. Here, we found KU complex abundantly expressed in the cytoplasm, where it recognized accumulated cytoplasmic DNA in aged human and mouse CD4 + T cells ...Ku and DNA-dependent protein kinase dynamic conformations and assembly regulate DNA binding and the initial non-homologous end joining complex. J. Biol. Chem., 285 (2010), pp. 1414-1423. View PDF View article CrossRef View in Scopus Google Scholar. Jakob et al., 2009.Ku proteins, in contrast, followed a pattern of trapping similar to that for Polβ: MMS failed to produce Ku-DPC, while treatment with CuOP or (less effectively) H 2 O 2 gave rise to significant Ku-DPC. Unexpectedly, NEIL1 and NEIL3 were trapped following H 2 O 2 treatment, but not detectably in cells exposed to CuOP. The half-life of all the ...This region absent in the x-ray structure of the Ku heterodimer with DNA (59) was shown to be flexible in solution by NMR determination and exhibited a high helical propensity, allowing folding upon Ku binding to DNA or to its protein partner (60, 61). Thus, Ku binding to DNA or the Ku80 C-terminal motif may displace Artemis from DNA-PKcs by ...Several proteins that are part of the multi-protein replication complex, but are not a member of the pre-RC, have a dual role in DNA replication and repair, such as PCNA (Dimitrova et al., 1999; Moldovan et al., 2007), the Replication Protein A (RPA)(Chesnokov, 2007) and the multifunctional Ku protein (reviewed in (Rampakakis et al., 2009a).

This region absent in the x-ray structure of the Ku heterodimer with DNA (59) was shown to be flexible in solution by NMR determination and exhibited a high helical propensity, allowing folding upon Ku binding to DNA or to its protein partner (60, 61). Thus, Ku binding to DNA or the Ku80 C-terminal motif may displace Artemis from DNA-PKcs by ...

Ku protein preparations (4 μg), prepared as stated above, were subjected to limited proteolysis by the addition of 200 ng of sequencing grade bovine trypsin. Reactions were performed in buffer A and incubated at 37°C for10 minutes. Reactions were terminated by the addition of SDS loading dye and samples were separated by SDS-PAGE.

The Protein Structure & X-Ray Crystallography Laboratory (PSXL) is a core laboratory at the University of Kansas that possesses the infrastructure and expertise to perform all aspects of protein and small molecule structure determination using X-ray crystallography. For protein structure determination, high-throughput methods are utilized to ...Jun 3, 2002 · A binding site for the telomere protein TRF2 is located in the N-terminal half of human Ku70 (Song et al., 2000) and, hence, the presence of a truncated Ku protein could potentially contribute to the unique telomere phenotype associated with ku70 mutants (see below). Requirement for the NHEJ pathway varies during plant developmentInteraction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids. Nucleic Acids Res. 1998;26:1551-1559. [PubMed] [DOI]. 8 ...The current thinking is that Ku protein binds to DNA discontinuously and in a sequence independent manner, carrying out a DNA-protective role . Once bound to DNA, Ku proteins recruit and activate the catalytic DNAPKcs subunit which can phosphorylate Ku and other neighboring DNA-bound proteins . It has also been reported that DNAPKcs self ...damage or co-precipitate Ku protein complexes if over-expressed. by itself as a GFP-fusion protein (Figs 2a and 3a; 'WRN-nA'). Mutation of the N-terminal KBM reduced Ku co-precipitation by.Autoantibodies against Ku are elicited by macromolecular protein complexes containing Ku and the associated DNA damage proteins may be involved of autoimmunity in rheumatic diseases. Expression of Ku70 in breast cancer tissue and normal breast tissue with immunohistochemistry are largely equal.The Ku heterodimer (Ku70/Ku80) plays a central role in DNA double strand break recognition and repair. It has been shown, more than ten years ago, that Ku is also expressed at the cell surface of ...We explore unique structural and functional aspects, detail Ku expression, conservation, and essentiality in different species, discuss the evidence for its involvement in a diverse range of cellular functions, highlight Ku protein interactions and recent work concerning Ku-binding motifs, and finally, we summarize the clinical Ku-related ... CA125 is a protein that is found in blood. A small sample of blood will be taken from your arm and sent to a lab where a simple test will measure the level of CA125 in your blood sample. In most healthy women the level of CA125 is usually less than 35. However, some women do have a naturally high level of CA125 in their blood.

Oct 22, 2009 · In NHEJ, DSBs are recognized by the Ku protein that then binds and activates the protein kinase DNA-PKcs, leading to recruitment and activation of end-processing enzymes, polymerases and DNA ...15-10-2023. PhD fellowship in Experimental Quantum Optics, Niels Bohr Institute. Faculty of Science. Niels Bohr institutet. 15-10-2023. 160061 Associate Professor of cognitive neurorehabilitation (Part time, Fixed-Term, 5 Years) Faculty of Health and Medical Sciences. Department of Neuroscience. 15-10-2023.Nov 12, 2021 · Collectively, our data show that zinc-finger protein ZNF384 is an adapter of Ku to DNA during DSB repair via cNHEJ. Results. ZNF384 is recruited to DNA damage sites and interacts with NHEJ proteins.The fact that Ku forms a ring to encircle duplex DNA explains why Ku requires DNA ends for high-affinity binding (Figs 1 and 2).Unlike the uniform, symmetrical protein rings observed in ...Instagram:https://instagram. citations in wordncaa poyciv 6 best map typeteam building powerpoint presentation The Ku protein has a critical function in the repair of double-strand DNA breaks induced for example by ionizing radiation or during VDJ recombination. Ku serves as the DNA-binding subunit of the DNA-dependent kinase and is a heterodimeric protein composed of 80- and 70-kDa subunits. We used the two-hybrid system to analyze the interaction ...The Ku protein has a critical function in the repair of double-strand DNA breaks induced for example by ionizing radiation or during VDJ recombination. Ku serves as the DNA-binding subunit of the DNA-dependent kinase and is a heterodimeric protein composed of 80- and 70-kDa subunits. We used the two-hybrid system to analyze the interaction ... kansas recyclingreasons to do online school Dynan, W. S. & Yoo, S. Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with ... References 103–107 identify RIF1 as a 53BP1 effector protein during DSB repair ...The human Werner syndrome protein, WRN, is a member of the RecQ helicase family and contains 3′→5′ helicase and 3′→5′ exonuclease activities. Recently, we showed that the exonuclease activity of WRN is greatly stimulated by the human Ku heterodimer protein. We have now mapped this interaction physically and functionally. ethnoculture The Ku protein is a complex of two subunits, Ku70 and Ku80, and was originally identified as an autoantigen recognized by the sera of patients with autoimmune diseases. The Ku protein plays a key role in multiple nuclear processes, e.g., DNA repair, chromosome maintenance, transcription regulation, …Jun 24, 2023 · The nuclear pore complex is the key regulator of transport between the cytoplasm and nucleus. Emerging evidence suggests it also regulates gene expression by influencing the internal architecture ...